Coding
IodoY RS

Part:BBa_K1416001:Design

Designed by: Jordan Monk   Group: iGEM14_Austin_Texas   (2014-10-06)


The tRNA synthetase/tRNA needed for incorporating 3-iodo-L-tyrosine (IodoY) at a UAG codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1153
    Illegal BamHI site found at 1159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1185
    Illegal NgoMIV site found at 1645
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316
    Illegal SapI.rc site found at 1109


Design Notes

Source

This part is from the genomic sequence of Methanocaldoccus jannaschii. Wang L, et al. Expanding the Genetic Code of Escherichia coli. Science 20 April 2001: 292 (5516), 498-500.

References